Kat. № T2-T
Model T2-T, where «T» stands for «Thrombin generation registration» (in addition to fibrin)
Thrombodynamics Analyser T2-T records and analyses spatiotemporal dynamics of thrombin generation simultaneously with spatiotamporal dynamics of formation / lysis of a fibrin clot.
Clotting starts from the localized coagulation activator and propagates in a thin layer of non-stirred blood plasma sample. The Thrombodyanmics Analyser System - T2-T model - analyses spatiotemporal dynamics of fibrin clot formation and thrombin generation and calculates numerical parameters describing the coagulation process.
Thrombodynamics Analyser (both T2-F and T2-T models) allows visualization of the fibrinolysis process in presence of specific activators such as tPA and uPA. These substances lead to dissolution of the fibrin clot after its formation with a specific pattern. Clot formation is activated by the immobilized TF in the cuvette and then the lysis can be observed.
An example of report of clot growth and further lysis:
In addition to registering fibrin clot growth and lysis from the immobilized coagulation activator, Thrombodynamics Analyser T2-T allows registering spatiotemporal dynamics of thrombin, the main enzyme of the coagulation cascade.
Registration of thrombin formation is based on fluorescent microscopy principle. Fluorogenic substrate for thrombin is added to plasma sample. The fluorogenic substrate is 7-amino-4-methylcoumarin (AMC) bound to a short amino acid sequence, which is required for recognition of substrate by thrombin. When bound to the substrate, AMC does not have an effect on plasma optical properties. As a result of substrate cleavage by thrombin, free AMC appears in plasma and fluoresces. The rate of AMC formation in each point is proportional to local thrombin concentration.
On the basis of the recorded photos of AMC fluorescence the Thrombodynamics Analytical Software calculates the numerical parameters of spatiotemporal dynamics of thrombin generation (thrombodynamics-4D parameters).
- Thrombodynamics Analyser Device, Model T2-T model
- Dimensions: 430x230x160 mm (device without notebook)
- Weight: 10 kg max (device without notebook)
- Power supply: 100 – 242 V AC, 50 – 60 Hz
- Power consumption: 230 VA max
- Sample volume: 0.12 ml
- Number of measuring channels: 2
- Number of preheated places: 4
- Personal Computer (Notebook) with Thrombodynamics Analytical Software installed
- Accessories and supplies:
- Mouse for notebook
- USB & LAN adapter
- Service kit
- User manual
Measurement type: Thrombodynamics (TD), Thrombodynamics-4D (TD-4D), Fibrinolysis
Measurement principle:
- Registration of light scattering signal from the growing fibrin clot
- Registration of fluorescence signal during cleavage of synthetic AMC-based substrate by thrombin
- 30 min for TD by default
- 60 min for TD4D by default
- Max duration - 8 hours
- 4 test/hour for TD measurements
- 2 test/hour for TD4D measurements
Brightness control: 0 to 255 a.u. for all channels simultaneously
